4rxn (fluidigm)

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fluidigm 4rxn

4rxn, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxpar+antibody+labeling+kit+x8+4rxn/Maxpar+X8+Antibody+Labeling+Kit%2C+151Eu/pmc11148643-105-6-8
Average 93 stars, based on 4 article reviews

4rxn - by Bioz Stars, 2026-10

93/100 stars

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1) Product Images from "Development of a chimeric cytokine receptor that captures IL-6 and enhances the antitumor response of CAR-T cells"

Article Title: Development of a chimeric cytokine receptor that captures IL-6 and enhances the antitumor response of CAR-T cells

Journal: Cell Reports Medicine

doi: 10.1016/j.xcrm.2024.101526

Figure Legend Snippet:

Techniques Used: Purification, Virus, Recombinant, Enzyme-linked Immunosorbent Assay, Staining, Antibody Labeling, Mass Cytometry, Mutagenesis, Software, Imaging

Bioprocessing:

Article Title: Combining Mass Cytometry Data by CyTOFmerge Reveals Additional Cell Phenotypes in the Heterogeneous Ovarian Cancer Tumor Microenvironment: A Pilot Study
Article Snippet: Metal-conjugated antibodies (n = 28) and purified monoclonal antibodies (n = 6) were purchased from Fluidigm (San Francisco, CA, USA), BioLegend (San Diego, CA, USA), and Abcam (Cambridge, MA, USA). .. All the in-house conjugated monoclonal antibodies were conjugated to pre-determined metal isotopes with the MaxPar Antibody Labeling Kit X8 4Rxn (Fluidigm, San Francisco, CA, USA) according to the producer’s protocol. .. Directly after this step, the conjugated antibodies were diluted to 1 μg/μL in Ab Stabilizer Solution (Candor Biosciences, Wangen, Germany) supplemented with 0.01% sodium azide (Santa Cruz Biotechnology, Dallas, TX, USA) and stored at 4 °C.

Article Title: Combining Mass Cytometry Data by CyTOFmerge Reveals Additional Cell Phenotypes in the Heterogeneous Ovarian Cancer Tumor Microenvironment: A Pilot Study.
Article Snippet: .. Antibody CONJUGATION and Validation All the in-house conjugated monoclonal antibodies were conjugated to pre-determined metal isotopes with the MaxPar Antibody Labeling Kit X8 4Rxn (Fluidigm, San Francisco, CA, USA) according to the producer’s protocol. .. Directly after this step, the conjugated antibodies were diluted to 1 μg/μL in Ab Stabilizer Solution (Candor Biosciences, Wangen, Germany) supplemented with 0.01% sodium azide (Santa Cruz Biotechnology, Dallas, TX, USA) and stored at 4 ◦C.

Antibody Labeling:

Article Title: Mass Cytometry Analysis Reveals the Landscape and Dynamics of CD32a + CD4 + T Cells From Early HIV Infection to Effective cART
Article Snippet: Antibodies (listed in Table in Supplementary Material) were either pre-conjugated from the manufacturer (Fluidigm, San Francisco, CA, USA) or conjugated in-house with the appropriate metal isotopes. .. Between 200 and 400 μg pure mAbs (carrier-protein-free) from various manufacturers were coupled to Lanthanide from the MaxPar Antibody Labeling Kit X8 4Rxn (Fluidigm). .. Conjugated Abs were adjusted to 1 μg/μl in Ab Stabilizer Solution (Candor, Biosciences, Wangen, Germany), supplemented with 0.01% sodium azide (Santa Cruz Biotechnology), and stored at 4°C.

Article Title: Combining Mass Cytometry Data by CyTOFmerge Reveals Additional Cell Phenotypes in the Heterogeneous Ovarian Cancer Tumor Microenvironment: A Pilot Study.
Article Snippet: .. Antibody CONJUGATION and Validation All the in-house conjugated monoclonal antibodies were conjugated to pre-determined metal isotopes with the MaxPar Antibody Labeling Kit X8 4Rxn (Fluidigm, San Francisco, CA, USA) according to the producer’s protocol. .. Directly after this step, the conjugated antibodies were diluted to 1 μg/μL in Ab Stabilizer Solution (Candor Biosciences, Wangen, Germany) supplemented with 0.01% sodium azide (Santa Cruz Biotechnology, Dallas, TX, USA) and stored at 4 ◦C.

Conjugation Assay:

Article Title: Combining Mass Cytometry Data by CyTOFmerge Reveals Additional Cell Phenotypes in the Heterogeneous Ovarian Cancer Tumor Microenvironment: A Pilot Study.
Article Snippet: .. Antibody CONJUGATION and Validation All the in-house conjugated monoclonal antibodies were conjugated to pre-determined metal isotopes with the MaxPar Antibody Labeling Kit X8 4Rxn (Fluidigm, San Francisco, CA, USA) according to the producer’s protocol. .. Directly after this step, the conjugated antibodies were diluted to 1 μg/μL in Ab Stabilizer Solution (Candor Biosciences, Wangen, Germany) supplemented with 0.01% sodium azide (Santa Cruz Biotechnology, Dallas, TX, USA) and stored at 4 ◦C.

Biomarker Discovery:

Article Title: Combining Mass Cytometry Data by CyTOFmerge Reveals Additional Cell Phenotypes in the Heterogeneous Ovarian Cancer Tumor Microenvironment: A Pilot Study.
Article Snippet: .. Antibody CONJUGATION and Validation All the in-house conjugated monoclonal antibodies were conjugated to pre-determined metal isotopes with the MaxPar Antibody Labeling Kit X8 4Rxn (Fluidigm, San Francisco, CA, USA) according to the producer’s protocol. .. Directly after this step, the conjugated antibodies were diluted to 1 μg/μL in Ab Stabilizer Solution (Candor Biosciences, Wangen, Germany) supplemented with 0.01% sodium azide (Santa Cruz Biotechnology, Dallas, TX, USA) and stored at 4 ◦C.

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